产品名称FEMTO3D Atlas & FEMTO3D Atlas Plug & Play

 

Breakthrough innovation in two-photon microscopy

The Innovation Award winner FEMTO3D Atlas combines high-tech science and engineering in 3D measurements. It implements and goes beyond the traditional galvo and resonant scanner-based imaging functions and combines them with a unique fast 3D imaging feature, providing an All-in-one solution in two-photon microscopy. The FEMTO3D Atlas enables its users to scan cells and neuronal, dendritic, or other biological processes in 3D, up to a million times faster than classical scanning methods, at a preserved two-photon resolution.

 

 

 

NETWORK IMAGING

Neural networks consist of thousands of neurons dispersed in 3D space, often across many cortical layers of the brain tissue. 

3D random-access scanning performed by AO technology combined with two-photon microscopy is the best choice to reveal the dynamics of the neural networks while processing information.

It enables you to record neuronal activity from cell populations consisting of up to thousands of cells in vivo.

The 3D anti-motion technology combined with new scanning methods united in FEMTO3D Atlas provides a flexible solution to gather motion-corrected data in large volumes at high framerates during behaviour.

 

 

DENDRITIC IMAGING

3D random-access point scanning extended by drifting the focal point along scanning elements with various shapes and sizes allows imaging without interruption at multiple dendritic branches.

The sampling is continuous during the drift, so this scanning mode gives more detailed spatial resolution without changing the overall scanning time which reminds as high as during point scanning. 

As a result, the function of thin dendritic segments or even spines, single action potentials can be brought to light. 

 

 

BEHAVIORAL STUDIES

Our anti-motion technology enables cell activity to be captured while an animal is moving in virtual reality and performing tasks.

To preserve signals while imaging, scanning points are extended to drifted lines which are precisely fitted to each other resulting in surface or volume elements enclosing the target object.

These elements cover not only the pre-selected ROIs but also the neighboring areas giving an opportunity for motion correction by preserving all fluorescent information during motions and decreasing the artefacts by more than one order of magnitude in behaving animals.

 

 

OPTOGENETICS

Photostimulation techniques allow activating cells in a selective and precise manner, which makes them advantageous for various biological applications.

Enhancing two-photon microscopy with this capability makes this technology well suited for studies ranging from synaptic plasticity to experiments investigating learning and memory in vivo in the brain tissue.

To the FEMTO3D Atlas Dichro extension, a second laser can be coupled enabling users to perform optogenetic stimulation or uncaging with calcium imaging, simultaneously, even in different cell populations.

 

 

 

3D REGION SCANNING

Using two-photon microscopy enhanced by the AO technology in combination with our easy-to-use data acquisition software package provides maximal flexibility in the fast selection of 3D ROIs with various shapes and sizes such as points, trajectories, squares, etc.

The preselected ROIs can be precisely and rapidly targeted in the tissue without wasting measurement time on unnecessary background areas, increasing the measurement speed to up to 100 kHz and the signal-to-noise ratio by several orders of magnitude in comparison to classical raster scanning.

 

 

3D REAL-TIME MOTION CORRECTION

The Femtonics 3D-RTMC provides quasi-instantaneous and simultaneous Real-Time Motion Correction on your in vivo recordings, ensuring immaculate recordings every time.

This real-time solution eliminates artifacts caused by heartbeat, breathing or physical activity at over 100 kHz speed allowing calcium and voltage imaging from spines, dendrites, axons and somata with high resolution.

With the implementation of 3D random-access line scanning, we can now correct motion along each axis.

Moreover, our approach offers the significant advantage of enabling Z directional (axial) correction just as fast, that surpasses the currently available XY plane cross-correlation-based solutions.

 

 

 

HIGH-SPEED FRAME SCANNING

The high-speed arbitrary raster scan mode of the two-photon microscopy based FEMTO3D Atlas provides a scanning speed of 40 fps at 510 x 510 pixels and 500 x 500 µm², which is faster than most resonant scanning two-photon microscopes, their highest speed being around 30 fps.

Thanks to the AO technology, the scanning plane can be arbitrarily chosen while the speed is being maintained: the plane being scanned with a high speed can be perpendicular to the axis of the objective, or lying at an arbitrary angle in X and/or Y.

 

 

3D PHOTOSTIMULATION

Using the 3D random-access excitation method of the two-photon microscopy based FEMTO3D Atlas Dichro, scanning methods of the Femtonics AO technology are available with flexible parameters for photostimulation.

Depending on the selected scanning pattern, users can stimulate sparsely distributed individual cells or dendritic processes in a large volume with high precision. 

By rapidly switching between the two laser lines, activity can be recorded near-simultaneously with photostimulation.

 

 

 

Atlas combines an intoxicating blend of high-tech science, engineering, refinement in 3D measurements.

 

FEATURES AND BENEFITS

---  Available both as a standalone microscope or as a 3D imaging extension for various types of existing system

---  Simultaneous read out of neural activity on both the somatic and dendritic scales

---  Increased measurement speed and signal-to-noise ratio by several orders of magnitude

---  3D Network imaging of over 2000 soma distributed in 3D

---  Imaging of dendritic branches through layers

---  Simultaneous imaging of over thousands of spines

---  Unique, flexible scanning methods supporting in vivo and in vitro measurements

---  In vivo real-time 3D motion correction 

---  High-speed 3D random-access ROI scanning: scanning speed up to 100 kHz to any points in 3D

---  High-speed raster scanning: 40 fps at 510×510, and even 3000 fps at reduced FOV

---  Improved excitation efficiency for large 3D scanning volume with GECIs

---  Automatic wavelength tuning between 750 – 1050 nm

---  Functional imaging correlated with electrophysiological data

---  Calcium and voltage imaging during behavior

---  3D random-access photo stimulation for optogenetics

 

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What is FEMTO3D ATLAS PLUG & PLAY?

The FEMTO3D ATLAS PLUG & PLAY microscope is a turnkey multiphoton solution: following a smooth delivery to the laboratory, it is ready to operate within an hour. The system can be easily relocated or moved between laboratories, adapting to your ever-changing needs. While compact in size, the microscope is equipped with the latest 3D acousto optic (AO) technology for ultra-fast in vivo 3D imaging and 3D photostimulation.

The FEMTO3D ATLAS PLUG & PLAY combines high­ tech science and engineering in 3D measurements. It performs and goes beyond all that galvo and resonant scanner based imaging can do and extends into three dimensions, providing the all-in-one solution in two-photon microscopy.

 

Ultra-fast, simultaneous 3D imaging and 3D photostimulation in a large volume with real-time motion correction: capture dendrites and whole networks at the same time! Scan silently, without mechanical restrictions!

 

 

 

 

FEMTO3D ATLAS PLUG&PLAY

The FEMTO3D ATLAS PLUG & PLAY microscope is a turnkey multiphoton solution: following a smooth delivery to the laboratory, it is ready to operate within an hour.

The system can be easily moved within and between laboratories, adapting to your ever-changing needs.

While compact in size, the microscope is equipped with the latest 3D acousto-optic (AO) technology for ultra-fast in vivo 3D imaging and 3D photostimulation.

 

 

 

1、3D-RTMC (Real-Time Motion Correction)

for behaving animals

/Compatible with FEMTO3D ATLAS/

Technology Overview

Enhance your in vivo measurements effortlessly with the Femtonics 3D-RTMC, which offers Real-Time Motion Correction along X,Y and Z (axial) direction, effectively eliminating motion artifacts.

Neuroscience relies more heavily on in vivo measurements of neuronal activity to study cognition, memory, CNS diseases, behaviour, neurochemistry, and many other aspects of the field. One of the bottlenecks of in vivo functional measurements is motion artifacts caused by the movement of the animal, respiration, circulation, or any of the numerous other sources. Here we offer a real-time solution for in vivo motion correction which eliminates artifacts caused by heartbeat, breathing or physical activity at over 100 kHz speed allowing calcium and voltage imaging from spines, dendrites, axons and somata with high resolution.

 

 

 

2、DICHRO EXTENSION

for flexible photostimulation

/Compatible with FEMTO3D ATLAS/

Technology Overview

The Dichro option of the ATLAS product line allows you to use two independent laser sources with two different wavelengths in the same ATLAS scanhead. Since the acousto-optic deflection depends on the wavelength of light, different driving of the acousto-optic crystals selects only one of the input beams to pass through the scanhead. As a result, the user is able to select for each approximately 30 (10 with upgrades) us long AO-cycle, which input beam to focus within the 3D space of the sample. In-depth protocol editor and MultiPulse generator windows let you control imaging and photo-stimulation down to the finest details.

 

 

 

3、GREEN ILLUMINATION

for vessel pattern visualization

/Compatible with FEMTO3D ATLAS and FEMTO SMART/

Technology Overview

Green illumination from an LED light source allows high-contrast visualization of blood vessels, taking advantage of that the highly concentrated heme content of the red blood cells is excitable in the spectral region of 300-650 nm. Using green illumination helps to navigate on the surface of any organs under in vivo conditions and position a patch pipette for bulk loading or patch clamping.

 

4、4D Beam Conditioning Unit

for high-precision experiments

/Compatible with FEMTO3D ATLAS/

Technology Overview

Beam stability is of the utmost importance, when it comes to accuracy in any imaging system. This is why we developed the 4DBCU (4D Beam Conditioning Unit), to efficiently deal with stabilization problems, and control the beam in an elegant and effective manner. This is a very efficient optional module for the FEMTO3D Atlas, one that will eliminate many optical artifacts, as well as prepare the beam for any specific application, such as deep functional imaging.

 

 

5、IN VITRO EXTENSION KIT

for cultured cells or acute brain slices

/Compatible with FEMTO3D ATLAS and FEMTO SMART/

Technology Overview

Imaging acute brain slices or cultured cells and tissues enables the user to study cells in a controlled environment, outside of a living organism. Gradient contrast illumination eases camera guided patch-clamping while transmitted fluorescence detectors enhance signal collection and SNR.

 

 

 

6、LED LIGHT SOURCE

for full-field photostimulation
/Compatible with FEMTO3D ATLAS and FEMTO SMART/

Technology Overview

Full-field illumination using a selected LED source allows molecules and cells to be stimulated over the whole FOV homogeneously. Combine this module with gated detectors to achieve millisecond switching between stimulation and imaging.

 

 

SOFTWARE

 

FEMTO3D Atlas

The FEMTO3D Atlas software:

• our measurement control and analysis software suite powering the FEMTO3D Atlas,
• supports a multitude of imaging workflows from fast planar imaging to patterned 3D photostimulation and simultaneous multicolor activity recording.

Find 3D scanning modes and other features on the Atlas Software product page.