产品名称Second Generation Bussey-Saksida Touch Screen Chamber for Mice

Second Generation Bussey Chamber developed for mice with Intelli-interface supports up to 20 chambers on one PC. On the strength of 10 years of feedback and development, the system is even more flexible and with more features.

A Decade of continuous development

​​First developed in the 2009 at Cambridge University, the Bussey Saksida Rodent Touch Screen Chambers are now used by over 300 different Research Groups in at least 26 countries. The Bussey-Saksida Chamber has always featured a unique trapezoidal wall shape in order to focus the animal's attention and facilitates the efficient and high-throughput cognitive evaluation of rodents. The chamber is easily reconfigured to a modular square arena with panels, levers, lights, and a range of other operators to accommodate any task like an operant chamber equipped with a traditional lever or nose-poke.

Evolution: Meet the New Bussey-Saksida Chamber

Chambers Include

• Trapezoid walls in a non-reflective finish, to keep the animals focus on the screen and reducing distraction away from the screen.
• Ultra-sensitive Touch Screen to consistently detect a mouse nose-poke to the touchscreen. Commercially available touchscreens are not sensitive enough to reliably detect every nosepoke.
• A reward trough that can be place opposite or in front of the screen. While most tasks require the reward trough to be opposite the screen, some tasks (e.g. Autoshaping) require the reward in front of the screen.
• Pellet or Liquid reward.
• Activity beams to detect when the animal is at the back or front of the chamber.
• House and IR lighting
• Core Intelli-interface

​Designed to be ‘plug n play’ and highly productive.

• Chambers installed on our Easy Install System, ready to wheel in your lab. Attach the PC and you are ready to go.
• Fully validated ready written Task Suites which come with training paradigms and testing guidance.
• ABET Cognition Software gives you programming power, data analysis, versatility, and flexibility; video and audio control also included.
• Virtual Interface enables the user to easily write their own scheduling and assess the data away from the lab.
• On-line training to get your lab quickly working with high productivity.

 

 

Easy Install System

Paradigms

The Bussey-Saksida chamber allows usage of standard, original, and customized paradigms. Standard Task Paradigms are available by arrangement with the University of Cambridge. Standard tasks include popular Tasks such as PD, PAL, 5CSRT, PRC, Location Discrimination, and many more. The rodent tasks range from simple to complex and are virtually identical to tasks used with NHP & human populations on the CANTAB system. All paradigms include training routines as well as the main experimental paradigm and the data analysis sets.

NHP/Human CANTAB Equivalent	Standard Tasks	Typical time to reach baseline	Example neural systems involved	Clinical area showing impairment
NHP	Pretraining to touch an image and initiate a trial (All task except Auto and 5CSRT)	1-2 weeks (e.g., 7-8 week old C57BL6J mice: 5 days)		Learning
Human/NHP	Pairwise / Visual Discrimination (PD) and Reversal Learning	Pretraining + 5-7 sessions test to reach base line	Prefrontal Cortex, Perirhinal Cortex, Anterior Cingulate, Posterior Cingulate, Medial Frontal Cortex, Striatum, Dopamine system, Cholinergic system, NMDA receptors. Mediodorsal Nucleus of the Thalamus	Huntington's, Schizophrenia, Parkinson's, Learning, Cognitive Flexibility, Executive Function.
Human/NHP	Paired-Associate Learning Task for Rodents (PAL)	Pretraining + 35-45 sessions to 70%	Hippocampus, Cholinergic system, NMDA Receptors, AMPA Receptors	Alzheimer's, Schizophrenia, Spacial Memory
NHP	Visuomotor Conditional Learning for Rodents (VMCL)	Pretraining + Approximately 20 sessions	Dorsal Striatum, Posterior Cingulate Cortex	Huntington's, Parkinson's
Human/NHP	5-choice Serial Reaction Time Task for Rodents (5CSRT)	Pretraining (ave 10 sessions) + 3 weeks to 80% @ 2 sec baseline	Prefrontal Cortex, Basal Forebrain, Cholinergic (Accuracy), Serotonin (Impulsivity), Noradrenaline (Distraction), Dopamine (Motivation)	Alzheimer's, Depression, Huntington's, Schizophrenia, ADHD, OCD
	Autoshaping for Rodents (Auto)	Several sessions (no pretraining required)	Ventral Striatum, Amygdala, Anterior Cingulate Cortex, Nucleus Accumbens Dopamine, Pedunculopontine Tegmental Nucleus, D1 and NMDA receptors	Huntington's
Human/NHP	Trial-unique Nonmatching-to-Location Task for Rodents (TUNL)	Pretraining + 6-24 Sessions to acquire the basic task	Hippocampus, Cholinergic system, NMDA Receptors, Prefrontal Cortex	Alzheimer's, Schizophrenia
NHP	Location Discrimination for Rodents (LD)	Pretraining + 10-20 sessions	Hippocampus, Neurogenesis	Alzheimer's, Schizophrenia
NHP	Extinction for Rodents (EXT)	Approximately 4 sessions training + a sessions days extinction	Infralimbic Cortex, Striatum and Amygdala	ADHD, OCD
Human	5-Choice Continuous Performance Test for Rodents (5C-CPT)	Approximately 13 sessions (based on training in 5-hole box) after training to 5-CSRTT	Dopamine, Serotonin, Cholinergic, Parietal, Muscarinic.	Schizophrenia, ADHD, OCD, Alzheimer's
NHP	Progressive Ratio and Effort Related Choice Task for Rodents (PR/ERC)	16 sessions from first habituation to reach stable PR performance	Dopamine	Motivation, Decision Making
Human	4-Choice Gambling Task for Rodents (4C-GT)	Pretraining + 4 sessions familiarizing with choices available, then 13 sessions testing	Dopamine, Serotonin	Bipolar Disorder, Gambling, Depression, OCD, ADHD, Parkinson's, Schizophrenia, Impulsivity
Human/NHP	Rodent Continuous Performance Task: Image for Rodents (rCPT)	Pretraining + Approximately 30 sessions	Cholinergic system, Anterior Cingulate Cortex	Schizophrenia, ADHD, OCD, Alzheimer's
Human	Delay Discounting for Rodents (DD)	Pretraining + approximately 13 sessions	Dopamine	Impulsivity, ADHD
NHP	Probabilistic Reversal Learning for Rodents (PRL)	Pretraining + Approximately 17 sessions	Serotonin	Depression

Features

• Write your own Tasks or use the 14 pre-written validated Tasks currently available, including all training paradigms as a turn-key solution.

• Easier animal handling with side or top loading as standard.

• Door with magnetic catches for quiet operation.

• Lower cost and more robust construction.

• Walls now in a lighter color that provides better contrast for the camera to capture the animal’s image or contour when tracking.

• Reward trough has a ‘pellet retrieval’ and dual liquid feed options plus a wider aperture to accommodate head-staged animals. Two alternative front plates offering different aperture heights as standard.

• Modular design to minimize reconfiguration to accommodate tethered animals and/or electrophysiology recording.

• The chamber floor is now matted to reduced glare from the house-light, again for better consistent image capture by a camera for observation or tracking. 

• Easier access to remove the floor for cleaning.

• These new non-reflective walls and floors will aid video tracking, for those customers seeking the extra information this can provide.

• IR and ambient lighting located under the chamber lid provide even lighting of the chamber whilst eliminating glare from the lid interfering with the camera picture.

• For Integration with electrophysiology, all contact points between animal and chamber (floor, reward trough etc) have optional electrical isolation coating to prevent movement artefacts occurring. 

• IR tracking beams now have an instant test facility as well as improved sensitivity and longevity

• Optional square modular walls to add levers and other modules if required.

• Slotted lids for tethered animals.

• Shockable floor

• Integration with other measures such as Optogenetics, Photometry, Video Tracking and Electrophysiology Recording.

• Third party systems can be integrated with TTL input and output lines, with low e.m.c. noise 80614EA chambers for integrating with Electrophysiology Recording

Specifications

Animal Working Area

• 46mm Wide at Feeder, 

• 238mm Wide at Screen x 170mm Deep (241.4 Sq. cm or 37.5 Sq. in)

• Working Area is 230mm in Height

Standard Environmental Cabinet

• Attenuation: Approx. 35dB

• Weight: 38 kg (empty)

• External Dimensions: 600mm Wide x 670mm Tall x 352mm Deep

• Internal Dimensions: 540mm Wide x 610mm Tall x 532mm Deep

Science

The Touch Screen has been used to develop a wide range of tasks, probing different areas of cognition, most of which are available ready written with programs and information to train the rodents to the task.

The design of the Touch Screen chamber and the tasks were both found to be crucial to get good and consistent results - comparable results have been achieved by labs all over the world using the Lafayette/Campden Touch Screen and these prewritten tasks. Implementation of the smooth trapeziodal walls and extra sensitive Touch Screens significantly reduced the training times and rodent performance – minimizing distraction and making sure all nosepokes to the screen are detected.

As well as being able to compare results with other rodent Touch Screen work, the chambers also lend themselves to comparing with both NHP and Human Touch Screen Tasks.

View our webinar on Using Touchscreen Operant Systems to Study Cognitive Behaviors in Rodents

Translational Science

Human CANTAB was developed at Cambridge University, UK, from well-known animal models of behaviour.  This has allowed the development of a range of cognitive tests proven to be sensitive to specific regions of the brain. These language free Human CANTAB tests have been further developed to make them suitable for monkey studies - incorporating as few changes as possible. 

Monkey CANTAB was developed by a group including Drs T.W. Robbins and A.C. Roberts at the Department of Experimental Psychology, University of Cambridge, England, to improve the comparative assessment from animals to humans. The result is a battery of tests that can be used to study cognition in both experimental primates in the laboratory and humans in the clinic.  Link to Monkey CANTAB Page 

Professors Tim Bussey and Lisa Saksida at the Cognitive Systems Neuroscience Laboratory, University of Cambridge, England have also developed rodent touchscreen chambers and tasks for rats and mice to again find tasks that are translational to human cognitive tests. 

An important consideration in the development translational tasks has been to maximise the likelihood that the same cognitive abilities are used by both animals and humans to perform each test. Consequently, complex tasks are broken down into their constituent elements and each element assessed independently of the next in a step-by-step progression through the test. Guiding a subject through the test in this way restricts the use of alternative strategies and permits an analysis of performance in terms of the cognitive components involved. Functional equivalence is suggested if the pattern of performance of animals and humans, at each stage of the test, is qualitatively similar and if comparable impairments are seen following damage to selective brain structures. A growing bibliography is building evidence that this is the case. 

Key Paper - Touchscreen cognitive testing: Cross-species translation and co-clinical trials in neurodegenerative and neuropsychiatric disease, Palmer et al, Neurobiology of Learning and Memory, 2021, 107443. 

One case in point concerns the Paired Associate Learning Task (PAL). In human studies early onset Alzheimer’s patients have shown significant deficiencies on this task. Humans with a DLG2 mutation have been found to be impaired on PAL. Likewise DLG2 knock out mice show impairment as shown here. [Nithianantharajah et al, Nature Neuroscience, 2013, 16] 

Several strains of rats and mice have been used in the touchscreen chambers, including transgenic and knock out animals. 

In a Lab Animal Review article it is also argued that the translational nature the tasks, the lack of aversive stimuli and the wide range of psychological constructs that can be evaluated, means the touchscreen method can yield significant 3Rs (Replacement, Reduction and Refinement) benefits.

Media

Product Brochures and Fliers

 

Bussey-Saksida Touch Screen Paradigms

Operant Touch Screen Advantage Program

Bussey-Saksida Touch Screen Chambers for Rodents

 

Research Papers and Bibliographies

 

Touch Screen Bibliography

 

Videos

 

Second Generation Bussey-Saksida Touch Screen System

Using Touchscreen Operant Systems to Study Cognitive Behaviors in Rodents

 

Accessories

Required Accessories

 

Precision Pellet Dispenser for 45mg Pellets
Model 80209A-45

Precision food dispenser with dispensing wheel that accommodates 14mg pellets.

Details

 

ABET Cognition Software
Model 89515

From writing schedules to analyzing data, ABET Cognition gives you the powerful and flexible tools necessary to control a network of touch screen chamber systems.

Details

 

Precision Liquid Feed Pump
Model 80204A

The rate of flow for the Precision Liquid Feed Pump is been calibrated to give precise delivery of liquids at delivery rates suitable for rewarding mouse, rat, and primate.

Details

Optional Accessories

 

Delay Discounting Task (DD) for Mice
Model 89552A

Adapted for mice. As a measure of impulsivity, the Delay Discounting Task (DD) measures how long the rodent is prepared to wait for a larger reward.

Details

 

Extinction (EXT) for Mice
Model 89547A

Adapted for mice, this is a simple, but powerful, test of behavioral inhibition.

Details

 

Probabilistic Reversal Learning (PRL) for Mice
Model 89553A

Adapted for mice. Designed to measure sensitivity to valence feedback information, PRL is sensitive to serotonergic manipulations, suggesting this test may be used to study depression-relevant behavior.

Details

 

4-Choice Gambling Task (4C-GT) for Mice
Model 89550A

Adapted for mice, this test is based on the Iowa Gambling Task, sensitive to serotonergic and dopaminergic agents.

Details

 

Open Reward Trough for Mice with Large Head-stages
Model 80614A-07-10

Designed to fit the trapezoid walls of the Bussey-Saksida touch screen chamber, this alternative reward trough allows mice to take liquid reward without having to nosepoke into the reward trough.

Details

 

Two-choice Paired/Visual Discrimination Reversal (PD) for Mice
Model 89540A

Adapted for mice, this reversal learning task requires inhibition of prepotent responses and is known to be dependent on the prefrontal cortex.

Details

 

Paired-Associate Learning Task (PAL) for Mice
Model 89541A

Adapted for mice, this is a hippocampal task. In humans, this task has proved to be highly effective for the early detection of Alzheimer's disease.

Details

 

Visuomotor Conditional Learning (VMCL) for Mice
Model 89542A

Adapted for mice, this is a habit or stimulus-response task in which the rodent learns a conditional rule.

Details

 

5-choice Serial Reaction Time Task (5CSRT) for Mice
Model 89543A

Adapted for mice, this task is sensitive to cortical manipulations, especially those involving prefrontal cortex, and is highly dependent on cholinergic transmission.

Details

 

Progressive Ratio and Effort Related Choice Task (PR/ERC) for Mice
Model 89549A

Adapted for mice, this touch screen task has been shown equivalent to those performed with levers or nose-poke holes in measuring motivation and reward-related decision making.

Details

 

Rodent Continuous Performance Task: Image (rCPT) for Mice
Model 89551A

Adapted for mice. In this Rodent CPT (Image) task, 5 different images are shown briefly, one at a time and in a random order. One of the images is designated the target stimulus. In order to obtain reward, the subject must touch the target stimulus and withhold from touching the non-target stimuli.

Details

 

Liquid Reward Trough for Mouse Touch Screen Chamber
Model 80614A-07-01

Reward trough for liquid feed/reward.

Details

 

Trial-unique Nonmatching-to-Location Task (TUNL) for Mice
Model 89545A

Adapted for mice, this is a working memory task. TUNL is a delayed nonmatching-to-place task that prevents the mediating strategies associated with the equivalent lever task.

Details

 

Location Discrimination (LD) for Mice
Model 89546A

Adapted for mice, this is a spatial memory task with a reversal learning component. It is sensitive to hippocampal lesions and associated with glutamate receptor regulation and signaling.

Details

 

Autoshaping (Auto) for Mice
Model 89544A

Adapted for mice, this task measures a Pavlovian response to the screen. This is a very rapidly administered test of simple classical conditioning that is dependent on a reward system centered on the ventral striatum.

Details

 

5-Choice Continuous Performance Test (5C-CPT) for Mice
Model 89548A

Adapted for mice, this go/no-go task measures both attentional and inhibitory systems within a single task paradigm, enabling the assessment of vigilance.

Details

 

Reward Trough for Mouse Touch Screen Chamber with Electrical Isolation
Model 80614A-07-03

Reward Trough for liquid feed using electronics with reduced electromagnetic emission and electrical isolation coating for better integration with electrophysiology recording.

Details

Related Products

 

Second Generation Bussey-Saksida Touch Screen Chamber for Rats
Model 80604A

The Second Generation Bussey Chambers with Intelli-interface supports up to 20 chambers on one PC. On the strength of 10 years of feedback and development, the system is even more flexible and with more features.

Details

 

Easy-Install System for Touch Screen Systems
Model 80604A-20-41

The Easy-install was developed for the Bussey-Saksida Touch Screen Systems due to the large number of cables and connections that these systems require.

Details

 

ABET Core Intelli-Interface for Easy Multi-Chamber Control
Model 81430

ABET Core Intelli-interface is a smart interface which controls all aspects of your chambers including touchscreen and computer-generated audio.

Detail