Record from multiple locations in a single animal or many animals simultaneously with a single system
Integrated with video tracking software
GFP based indicators such as GCaMP6
RFP based indicators such as R-CaMP2 (optional)
By using a CCD camera through a fiber bundle, the channel number can be easily expanded without extra equipment. The single wavelength Multi-Fiber Photometry System is a camera based system that images one end of a patch cable containing a bundle of fibers and measures the photometry signal from the individual fibers in the bundle via image processing techniques. The other end of the patch cable consists of individual fiber branches that are typically connected to fiber stubs implanted into individual brain regions of interest or potentially even implanted into multiple subjects. An excitation light source is projected onto the bundled end of the cable and the excitation light travels down each individual branch to its respective brain region. Fluorescent reporters expressed in each brain region absorb the excitation light and emit an activity dependent light signal that travels back up the fiber branches to be imaged by the sensor.
Coupled With CineLyzer
The single wavelength Multi-Fiber Photometry System is an optional subsystem that is integrated into the CineLyzer behavioral tracking and analysis system. The CineLyzer system provides for digital video recording of the subject along with speed, position, and orientation tracking. It allows you to define arenas and zones and compute statistical information on the subject’s entry into, exit from, or dwell time within these zones or sequences of zones. Dynamic zones centered on the subject can also be used to analyze social interaction between subjects. Thus incorporating photometry into the CineLyzer environment provides the information necessary to make correlations between the photometry signal(s) and the subject’s performance on behavioral tasks.
How is Plexon’s photometry system different from traditional systems?
The genetically encoded calcium indicators (GECIs), such as GCaMP** proteins, enable optical monitoring of the neural activity in real time. Fiber photometry provides the most sensitive and easiest way to record neuronal activities of the deep brain structures in behaving animals. Traditional systems employ a single multimode optical fiber implanted in the brain, a beam splitter that separates the excitation light and the fluorescent signals of neuronal activity indicators returning through the same optical fiber, and a photodetector. This approach allows for imaging of one fiber at a time. Measuring multiple brain regions is crucial to investigate connection between different brain regions to produce behaviors and the neural correlates of social interaction. Plexon’s camera-based approach provides a cost effective means to image from multiple fibers with the same detector.
GCaMP is a genetically encoded calcium indicator that allows researchers to visualize changes in intracellular calcium in vivo. GCaMP can be used to tag cell-type specific neurons with engineered fluorescent proteins and then measure the levels of fluorescence in response to changes in intracellular calcium concentration. The fluorescence emission of the GCaMP indicator increases with elevating calcium concentration. Fiber photometry enables the researcher to record the neural activity of genetically defined subpopulations of neurons through an optic fiber in freely moving animals.
I am interested in recording from multiple animals simultaneously. Is this possible?
Yes, the branching patch cable terminates in 4 magnetic LC ferrules. Each of these ferrules can be attached to fiber stubs on different animals.
What types of behavioral tasks can be used with CineLyzer and photometry?
Elevated plus maze
Novel object recognition
Radial arm maze
Other behavioral paradigms